|
KMID : 0364219910340030394
|
|
Korean Journal of Zoology
1991 Volume.34 No. 3 p.394 ~ p.402
|
|
|
Synthesis and Fate of Yolk Protein-3 in Hyphantria cunea D.
|
|
Lee Sang-Dae
Kim Hak-Ryul
|
|
|
Abstract
|
|
|
|
Yolk protein-3 (YP3) was purified from the ovary of Hvpharatria cunea D. and the synthesis and fate during embryogenesis of WP3 were investisated by electrophoresis and fluorography. YP3 purified through gel slice and electrophoretic elution'was determined to have M. W. of 18 Kd and consist of one subunit. Haemolymph and fat body of male and female %were electrophoresed during vifellogenic stages to indentifv the vitellosenin in female. The result showed that there was no distinct difference in electrophoretic patterns betweerl male and female. However, tissue culture of fat body and maturing ovary indicated that YP3 was svuthesized by fat body. Aiso, vP3 in iaid eggs was maintained constant untii naut s artier oviposition and then decreased, indicating that YP3 was drastically used during late embryosenesis. However, a part of YP3 was present even in newly hatched first instar larvae.
|
|
|
KEYWORD
|
|
|
Hypgantria cunea, Yold protein, Embryogenesis, Electrophoresis, Fluorography
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|